In the present study a simple precise, rapid and reproducible reverse phase HPLC method has been developed and validated and the degradation behaviour of acyclovir under different stress conditions were performed. The chromatographic separation was achieved by using reverse phase stainless steel column of 4.6Ã?â??250 mm hypersil C18 HPLC column with 5 Ã?µm (particles packing) and mobile phase consists of a mixture of acetonitrile and 0.05M ammonium acetate in the ratio of 2:98 v/v. Flow rate of 1.5 ml per minute and injection volume 20 ?l was maintained. Elute was analyzed by a UV detector set at 251 nm. The retention time was found to be 7.55 min. The method is validated with respect to linearity, precision and robustness. The correlation coefficient was found to be 0.998 with relative standard deviation less than 2%. Acyclovir was found to degrade in all studied conditions but the extent of degradation was different. The method was completely validated showing satisfactory data for all method validated parameters tested. The developed method can be used for the stability indicating samples.
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